β-Amyloid (25-35)
同義詞：β-淀粉樣蛋白肽 (25-35)；Aβ25-35；β-淀粉樣蛋白肽 (25-35)
分子量：1060.27
分子式：C45H81N13O14S
CAS 號(hào)：131602-53-4
三字母：Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met
單字母：GSNKGAIIGLM
產(chǎn)品描述：
β-Amyloid (25-35)（Amyloid beta-peptide (25-35)）是阿爾茨海默氏淀粉樣蛋白β肽的Aβ(25-35)片段，在培養(yǎng)細(xì)胞中顯示出神經(jīng)毒性活性。
體外研究：
The amino acid sequence of Aβ(25-35) peptide is NH2-Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met-COOH, where the first Gly represents the amino acid 25 and the last Met represents the amino acid 35. Amyloid beta-peptide(25-35) is also investigated in gel state for the first time. Comparative studies are also carried out using vibrational absorption and ECD. The conformational preference of Aβ(25-35) peptide film is also investigated using vibrational absorption and VCD spectroscopy[1]. Amyloid beta-peptide(25-35) induces apoptotic effects on isolated brain mitochondria and the redox state of methionine-35, plays a key role in the induction of programmed cellular death pathways and toxic events[2].
β-Amyloid Aggregation Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Solid Aβ peptide was dissolved in cold hexafluoro-2-propanol (HFIP). The peptide was incubated at room temperature for at least 1h to establish monomerization and randomization of structure.
2. The HFIP was removed by evaporation, and the resulting peptide was stored as a film at -20 or -80 ℃.
3. The resulting film was dissolved in anhydrous DMSO at 5 mM and then diluted into the appropriate concentration and buffer (serum- and phenol red-free culture medium) with vortexing.
4. Next, the solution was age 48h at 4-8 ℃. The sample was then centrifuged at 14000g for 10 min at 4-8 ℃; the soluble oligomers were in the supernatant. The supernatant was diluted 10-200-fold for experiments.
Methods vary depends on the downstream applications.
體內(nèi)研究：
β-Amyloid 大量沉積，使神經(jīng)元內(nèi)的 Ca2+ 濃度升高，而 Ca2+ 又參與調(diào)控Tau 蛋白磷酸化，Tau 蛋白的過度磷酸化造成了神經(jīng)纖維纏結(jié)和神經(jīng)元死亡，最后動(dòng)物逐漸出現(xiàn)認(rèn)知癥狀，并發(fā)展為阿爾茨海默病。
