描 述:A continuous fluorometric assay employing Ac-DEVD-AMC has been developed for the measurement of caspase-3, a cysteine protease also called apopain, Yama or CPP-32. The design of this substrate Ac-DEVD-AMC was based on the substrate motif that has been used successfully with caspase-1, using the poly(ADP-ribose) polymerase cleavage site P₁-P₄ tetrapeptide.采用Ac-DEVD-AMC的連續(xù)熒光測定已開發(fā)用于測量半胱氨酸蛋白酶caspase-3,也稱為apopain, Yama或pcp -32。該底物Ac-DEVD-AMC的設(shè)計基于已成功用于caspase-1的底物基序,使用聚(adp核糖)聚合酶裂解位點P₁-P₄四肽。